280 Has-circ-0040626 promotes cell proliferation by sponging mir-126-3p and enhancing expression of ptchd4 in human umbilical vein endothelial cells

Kaposi sarcoma is characterized by increased proliferation of vascular and lymphatic endothelial cells. The roles circle RNAs playing not clear. Here, we explored the competing endogenous (ceRNA) mechanism from tissues cell in (KS). These (tumors compared to adjacent non-tumor tissue) were analyzed SBC Human ceRNA microarray. results are validated independent experiments quantitative PCR. And has-circ-0040626 overexpressed umbilical vein cells (HUVEC) that related miRNAs tested though tagged RNA affinity purification (TRAP) sequencing. We predicted target genes differential expressed next-generation sequencing GSE147704 Gene Expression Omnibus (GEO). In all, 541 differentially circRNAs (>2-fold, p value <0.05) found. significantly upregulated (1.47-fold, qPCR, which promotes HUVECs sponges miR-126-3p vitro. our previous study dataset GEO, patched domain containing 4 (PTCHD4) up-regulated tumor tissue all kinds KS, may be important for mechanism. concordant changes gene expression reveal promote through sponging targeting PTCHD4, a key early progression tumorigenesis KS.